We humans have always looked for ways for longer lifespan & be healthy till the last day of our lives. This would have felt like a dream a few years back but now we have science, research & evidence that clearly tells us that we can control our longevity.

Immortality is not something that can be achieved but we can certainly deep dive into what is happening inside our body at molecular & cellular level & look at improving our biology with right nutritional therapeutics.

The DNA- longevity mismatch

A multitude of external factors impact our lifespan & healthspan with nutrition playing a key role. However DNA does not have an enabler role to play in controlling our longevity. DNA( genes)  just  tell your potential of living a long & healthy life but it’s your lifestyle & external environment you are exposed to define your longevity.

The actual biological storyline- work your cells perform to extract & generate energy, repair, divide, fight etc is defined by how these genetic alphabets or genes are expressed based on what we decide to do everyday. 

Factors such as nutrition, sleep, exercise, stress, exposure to the environment like could alter your gut & oral microbial genes expression. It is these external factors that activate/express certain microbial & human genes that code for proteins that perform, inhibit or activate certain biochemical processes or functions(DNA-RNA-Protein).These functions could downregulate or upregulate certain molecular & cellular pathways & secrete metabolites into the bloodstream that can either trigger inflammatory cascades or deliver nutrients, neurotransmitters & program our biology for health or disease & make us age slower or faster relative to chronological age.

 This alteration in biochemistry does a great job in pinpointing what specific pathways are making us age slower or faster & what  kind of lifespan we are going to have.

Gut & Oral microbiome functions driving the biological age score

Since the beginning, we at Genefitletics have been passionate about deciphering how microbial  molecular & cellular functions(not their taxonomy or diversity) could alter & program our biochemistry for health & disease.

There are thousands of peer reviewed research journals  that clearly establish evidence behind the role of gut & oral microbiome in impacting digestion, immune functions, trigger chronic & systemic inflammation & result in onset of multiple chronic diseases & cancer. Although the science behind microbiome is really complex, we are now understanding the deep rooted microbiome-disease relationship.

What oral & gut microbes do in our body is really complex & fascinating. The interaction between our external environment & these tiny little bugs can stimulate changes in our biochemistry that are directly reflected in our body organs, tissues, blood, heart & brain.

ORAHYG Pro– Our full body biochemistry test uses your raw material-spit of your saliva to understand these interactions & how they translate into microbial genes expression as well as mitochondrial efficiency. This  empowers us to measure these changes at molecular & cellular level that are impacting your biological age.

The current practice of assessing biological age is restricted to blood telomere testing, DNA methylation or based on certain few blood biomarkers. However these techniques do not provide a comprehensive view of an individual’s biochemistry at molecular & cellular level & cannot identify molecular mechanisms that regulate biological age.

Deciphering your molecular storyline

Using ORAHYG Pro, we could translate the molecules extracted from your biological sample into mammoth genes expression data. This enables our machine learning model to transform huge ~ 10 million of molecular data into hundreds of molecular & cellular pathways that measure levels of multiple inflammatory & oxidative stress markers, stress response, mitochondria efficiency & cellular signalling molecules. Each of these pathways are scored to give you biological pathways & full body scores encompassing 400 plus scores. These pathways when combined mathematically truly reflect cellular decay, inflammation, oxidative stress & mitochondria biogenesis contributing to biological age. 

Introducing Agegorithm- Unique biological age test score for Indians based on their molecular & cellular biochemistry

With focus on prevention & healthier lifespan, we are now introducing a biological age test feature- Agegorithm to our existing full body biochemistry solution. Beginning March 2025, you could get your biological score relative to your chronological age based on your molecular & cellular pathways.

The  score will tell you how your body is aging at cellular level. The good part is while your chronological age is ticking, the biological age is dynamic & keeps on moving up or down based on upregulation or downregulation of cellular & molecular pathways driven by your environment & nutrition choices. This is where Genefitletics comes in.

With our biological age test feature, we do not look at a few hundred blood biomarkers, DNA methylation or blood telomere markers. Rather we look at tens of thousands of molecular interactions in every single pathway for 400 plus pathways to assess the impact of each of the inflammatory & oxidative stress markers on microbial cells & mitochondria. We have built a machine learning driven biological score equation, validated based on our clinical study(discussed below), that mathematically combines 400 plus pathways to determine your biological age score. This score is included as one of the many molecular insights you get with ORAHYG Pro.

We can then marry expression level of pathways with our nutrition ontology to construct & let you know what specific nutritional ingredients your microbiome & cells need to reinstate cellular homeostasis & slow down aging.

Clinical study on 2 billion molecular data sets

To validate the hypothesis of our machine learning driven biological age equation( model), we conducted a clinical study on 2 billion molecular data sets coming from 66 subjects. We bifurcated these 66 subjects into the discovery cohort (47, healthy controls) & validation cohort(19, diseased subjects ). We then looked at the mean biological age score for each of the pathways for both the discovery & validation cohort groups & calculated the expression level of each of the assigned pathways. 

Graphical representation of some of pathways expression level

We then calculated P value of each of the pathways of validation group & then adjusted it using Benjamini-Hochberg test.

Graphical representation of P value of molecular pathways

Using adj P value with threshold of P value< 0.05, we determined which specific pathways were significant which were included for the biological age score model. Our statistical analysis showcased that our hypothesis for biological age score is true. 

For those interested in learning more about research, you could express your interest here

Final words

Unlocking the future of longevity has become one of key proponents of biohacking for healthcare professionals, biohackers & individuals. While analysing gene expression & microbiome functions were considered a distant dream a few years back, the advent of exponential technologies & their integration with system biology has made it possible now at scale. It is no longer an alien world. The future is here now &  just a spit of saliva gives you access to your personalised actionable insights & molecular pathways & associated interventions to reinstate your metabolic & cellular homeostasis.

Your chance to improve your aging & longevity with the right set of interventions for food, supplements, biotics, food extracts, herbs & more in precise quantity & frequency based on analysis of your unique biochemistry at molecular level is just a click away.

The Biological age test score will be made available from March 2025 onwards. In case you’d want to sign up for ORAHYG PRO, you could hop on a free discovery call or chat on whatsapp with us.